|
KMID : 1134820130420081197
|
|
Journal of the Korean Society of Food Science and Nutrition
2013 Volume.42 No. 8 p.1197 ~ p.1203
|
|
|
Effects of Endurance Exercise and Ginsenoside Rb1 on AMP-Activated Protein Kinase, Phosphatidylinositol 3-Kinase Expression and Glucose Uptake in the Skeletal Muscle of Rats
|
|
Jung Hyun-Lyung
Shin Young-Ho
Kang Ho-Youl
|
|
|
Abstract
|
|
|
|
This study investigated the effects of endurance exercise and ginsenoside Rb1 on AMP-activated protein kinase (AMPK), phosphatidylinositol 3-kinase (PI3K) protein expression and glucose uptake in the skeletal muscle of rats. A total of 32 rats were randomly divided into four groups: CON (Control group, n=8), Ex (Exercise group; 25 m/min for 1 h, 6 days/week, 2 weeks, n=8), Rb1 (Ginsenoside Rb1 group; n=8), and Rb1/Ex (Rb1+Exercise group, n=8). The Rb1 and Rb1/Ex groups were incubated in ginsenoside Rb1 (KRBP buffer, 100 μg/mL) for 60 min after a 2-week experimental treatment. After 2 weeks, the expression of phosphorylated AMPKα Thr172, total AMPKα, the p85 subunit of PI3K, pIRS-1 Tyr612, and pAkt Ser473 were determined in the soleus muscle. Muscle glucose uptake was measured using 2-deoxy-D-[3H] glucose in epitroclearis muscle. Muscle glucose uptake was significantly higher in the three experimental groups (Ex, Rb1, Rb1/Ex) compared to the CON group (P<0.05). The expression of tAMPKα and pAMPKα Thr172 was significantly higher in the Ex, Rb1, and Rb1/Ex groups compared to the CON group (P<0.05). The expression of pAkt Ser473 was significantly higher in the Rb1 group compared to the CON and EX groups. However, the expression of pIRS-1 Tyr612 and the p85 subunit of PI3K were not significantly different between the four groups. Overall, these results suggest that ginsenoside Rb1 significantly stimulates glucose uptake in the skeletal muscle of rats through increasing phosphorylation in the AMPK pathway, similar to the effects of exercise.
|
|
|
KEYWORD
|
|
|
exercise, ginsenoside Rb1, muscle glucose uptake, AMPK
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|